Nuclear transfer into mouse zygotes

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 2000 English Publisher:Springer Science and Business Media LLCJournal:Nature Genetics, volume 24, pages 108-109 (issn: 1061-4036, eissn: 1546-1718,

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Authors: T, Wakayama; H, Tateno; P, Mombaerts; R, Yanagimachi;

doi: 10.1038/72749

pmid: 10655051

Nuclear transfer into mouse zygotes

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Abstract

We have previously reported a novel nuclear-transfer technique to clone mice from adult somatic cells1, 2 and late-passage embryonic stem cells3. The method entails the injection of a somatic-cell nucleus into an enucleated, unfertilized oocyte using a piezo-electrically driven micromanipulator. Embryonic development is activated by incubation with strontium chloride one to six hours after nuclear transfer.

Related Organizations

Rockefeller University
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University of Hawaii at Manoa
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University of Hawaiʻi Sea Grant
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University of Hawaii System
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New York University
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Keywords

Cell Nucleus, Nuclear Transfer Techniques, Zygote, Mice, Inbred Strains, Chromosomes, Mice, Blastocyst, Karyotyping, Oocytes, Animals, Female

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