Crystal structure of a plant catechol oxidase containing a dicopper center
Copyright policy )Crystal structure of a plant catechol oxidase containing a dicopper center
Catechol oxidases are ubiquitous plant enzymes containing a dinuclear copper center. In the wound-response mechanism of the plant they catalyze the oxidation of a broad range of ortho-diphenols to the corresponding o-quinones coupled with the reduction of oxygen to water. The crystal structures of the enzyme from sweet potato in the resting dicupric Cu(II)-Cu(II) state, the reduced dicuprous Cu(I)-Cu(I) form, and in complex with the inhibitor phenylthiourea were analyzed. The catalytic copper center is accommodated in a central four-helix-bundle located in a hydrophobic pocket close to the surface. Both metal binding sites are composed of three histidine ligands. His 109, ligated to the CuA site, is covalently linked to Cys 92 by an unusual thioether bond. Based on biochemical, spectroscopic and the presented structural data, a catalytical mechanism is proposed in which one of the oxygen atoms of the diphenolic substrate binds to CuB of the oxygenated enzyme.
- The University of Texas System United States
- Texas A&M University United States
- University of Münster Germany
Models, Molecular, Binding Sites, Protein Conformation, Molecular Sequence Data, Plants, Crystallography, X-Ray, Phenylthiourea, Hemocyanins, Animals, Humans, Amino Acid Sequence, Enzyme Inhibitors, Oxidation-Reduction, Sequence Alignment, Catechol Oxidase, Copper, Solanaceae
Models, Molecular, Binding Sites, Protein Conformation, Molecular Sequence Data, Plants, Crystallography, X-Ray, Phenylthiourea, Hemocyanins, Animals, Humans, Amino Acid Sequence, Enzyme Inhibitors, Oxidation-Reduction, Sequence Alignment, Catechol Oxidase, Copper, Solanaceae
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).828 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 0.1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 0.1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!