A minor groove RNA triple helix within the catalytic core of a group I intron
Copyright policy )A minor groove RNA triple helix within the catalytic core of a group I intron
Close packing of several double helical and single stranded RNA elements is required for the Tetrahymena group I ribozyme to achieve catalysis. The chemical basis of these packing interactions is largely unknown. Using nucleotide analog interference suppression (NAIS), we demonstrate that the P1 substrate helix and J8/7 single stranded segment form an extended minor groove triple helix within the catalytic core of the ribozyme. Because each triple in the complex is mediated by at least one 2'-OH group, this substrate recognition triplex is unique to RNA and is fundamentally different from major groove homopurine-homopyrimidine triplexes. We have incorporated these biochemical data into a structural model of the ribozyme core that explains how the J8/7 strand organizes several helices within this complex RNA tertiary structure.
- Yale University United States
Models, Molecular, Base Sequence, Molecular Sequence Data, Introns, Models, Chemical, Catalytic Domain, Tetrahymena, Animals, Nucleic Acid Conformation, RNA, Catalytic, RNA, Protozoan
Models, Molecular, Base Sequence, Molecular Sequence Data, Introns, Models, Chemical, Catalytic Domain, Tetrahymena, Animals, Nucleic Acid Conformation, RNA, Catalytic, RNA, Protozoan
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