Auxin regulates SCFTIR1-dependent degradation of AUX/IAA proteins
Copyright policy )Auxin regulates SCFTIR1-dependent degradation of AUX/IAA proteins
The plant hormone auxin is central in many aspects of plant development. Previous studies have implicated the ubiquitin-ligase SCF(TIR1) and the AUX/IAA proteins in auxin response. Dominant mutations in several AUX/IAA genes confer pleiotropic auxin-related phenotypes, whereas recessive mutations affecting the function of SCF(TIR1) decrease auxin response. Here we show that SCF(TIR1) is required for AUX/IAA degradation. We demonstrate that SCF(TIR1) interacts with AXR2/IAA7 and AXR3/IAA17, and that domain II of these proteins is necessary and sufficient for this interaction. Further, auxin stimulates binding of SCF(TIR1) to the AUX/IAA proteins, and their degradation. Because domain II is conserved in nearly all AUX/IAA proteins in Arabidopsis, we propose that auxin promotes the degradation of this large family of transcriptional regulators, leading to diverse downstream effects.
- Commonwealth Scientific and Industrial Research Organisation Australia
- The University of Texas at Austin United States
- White Rose Consortium: University of Leeds; University of Sheffield; University of York United Kingdom
- University of York United Kingdom
SCF UBIQUITIN-LIGASE, RUB1, Recombinant Fusion Proteins, Amino Acid Motifs, Arabidopsis, PATHWAY, Gene Expression Regulation, Plant, YEAST, TRANSCRIPTION, Peptide Synthases, RESPONSE ELEMENTS, Growth Substances, Glucuronidase, Plant Proteins, NEDD8, SKP Cullin F-Box Protein Ligases, Indoleacetic Acids, Arabidopsis Proteins, Ubiquitin, Nuclear Proteins, Plants, Genetically Modified, GENE, FAMILY, Mutation, ARABIDOPSIS-THALIANA, Protein Binding, Transcription Factors
SCF UBIQUITIN-LIGASE, RUB1, Recombinant Fusion Proteins, Amino Acid Motifs, Arabidopsis, PATHWAY, Gene Expression Regulation, Plant, YEAST, TRANSCRIPTION, Peptide Synthases, RESPONSE ELEMENTS, Growth Substances, Glucuronidase, Plant Proteins, NEDD8, SKP Cullin F-Box Protein Ligases, Indoleacetic Acids, Arabidopsis Proteins, Ubiquitin, Nuclear Proteins, Plants, Genetically Modified, GENE, FAMILY, Mutation, ARABIDOPSIS-THALIANA, Protein Binding, Transcription Factors
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).1K popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 0.1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 0.1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.1%
Citations provided by BIP!Popularity provided by BIP!