Plasmid RP4 as a vector replicon in genetic engineering
Copyright policy )Plasmid RP4 as a vector replicon in genetic engineering
THERE is now considerable interest in the construction of plasmids containing DNA fragments from a variety of bacterial genera, for purposes of studying the expression of the genetic potentialities of the incorporated bacterial DNA in different bacterial hosts. We report that the self-transmissible plasmid RP4 has only one site susceptible to cutting by the enzyme EcoRI, and should therefore be a very suitable bacterial DNA vector in such experiments.
DNA, Bacterial, Electrophoresis, Agar Gel, Conjugation, Genetic, Centrifugation, Density Gradient, Extrachromosomal Inheritance, DNA, Single-Stranded, Carbon Radioisotopes, DNA Restriction Enzymes, Genetic Engineering, Plasmids
DNA, Bacterial, Electrophoresis, Agar Gel, Conjugation, Genetic, Centrifugation, Density Gradient, Extrachromosomal Inheritance, DNA, Single-Stranded, Carbon Radioisotopes, DNA Restriction Enzymes, Genetic Engineering, Plasmids
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