IN further attempts to find a solvent for melanin which would be of use in histological work, the following substances have been found to dissolve, in the cold, the pigments extracted from the ink sac of Sepia officinalis and melanoma tissue, and those prepared from adrenaline and 3,4-dihydroxyphenylalanine (dopa): ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, and ethylene glycol monobutyl ether. The monoethyl ether had the greatest solvent power. These melanins have also been found to be soluble, in the cold, in mixtures of ethyl lactate and benzyl alcohol, the best proportions being 6 of the former to 4 of the latter (by volume). In all these solvents the solubilities of sepia, melanoma and adrenaline melanins were, so far as could be determined from what must be regarded as impure specimens, of the same order, but the melanin from 3,4-dihydroxyphenylalanine was much less soluble than the others. However, these solvents failed to dissolve the melanin in sections of melanoma tissue, even with the aid of heat. The method used for extracting the sepia and melanoma melanins was: (1) solution in 10 per cent aqueous sodium hydroxide, (2) filtration and precipitation of the melanin by dilute hydrochloric acid, (3) removal of extraneous fatty substances by extraction with ethyl alcohol, chloroform and benzene, (4) repetition of (1) and (2). This extraction procedure was also applied to melanins from 3,4-dihydroxyphenylalanine and adrenaline, but in spite of this uniformity of treatment the former product was still the least soluble of the four types of pigment.