Preparation of Cell-free Tryptophanase

descriptionPublicationkeyboard_double_arrow_right Article 18 Jan 1947 English Publisher:Springer Science and Business Media LLCJournal:Nature, volume 159, pages 99-99 (issn: 0028-0836, eissn: 1476-4687,

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Authors: E A, DAWES; J, DAWSON; F C, HAPPOLD;

doi: 10.1038/159099a0

pmid: 20281233

Preparation of Cell-free Tryptophanase

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Abstract

IN our earliest studies on the tryptophanase complex1 active non-viable suspensions of E. coli were used. These had been sterilized by shaking with chloroform, 1 volume to 2 volumes of suspension. The mixture was shaken vigorously to 200 r.p.m. for 4 hours.

Keywords

Tryptophanase, Humans

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