descriptionPublicationkeyboard_double_arrow_right Article 01 May 2001 English Publisher:Springer Science and Business Media LLCJournal:Plant Molecular Biology, volume 46, pages 161-170 (issn: 0167-4412, eissn: 1573-5028,

Authors: Alicja Ziemienowicz; Barbara Hohn; You-Qiang Wu;

doi: 10.1023/a:1010679901911

pmid: 11442056

Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana

- Summary
- Subjects
- Metrics

Abstract

Here we report the purification and biochemical characterization of recombinant Arabidopsis thaliana DNA ligase I. We show that this ligase requires ATP as a source for adenylation. The calculated Km [ATP] for ligation is 3 microM. This enzyme is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates, but not in oligo(dT)/poly(rA) substrates. Double-stranded DNAs with cohesive or blunt ends are also good substrates for the ligase. These biochemical features of the purified enzyme show the characteristics typical of a type I DNA ligase. Furthermore, this DNA ligase is able to perform the reverse reaction (relaxation of supercoiled DNA) in an AMP-dependent and PPi-stimulated manner.

Related Organizations

Friedrich Miescher Institute
Switzerland
University of Gdańsk
Poland

Keywords

Adenosine Triphosphate, Base Sequence, DNA Ligases, Hydrolysis, Arabidopsis, Electrophoresis, Polyacrylamide Gel, Recombinant Proteins, DNA Primers, Substrate Specificity

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	16
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%