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Heme metabolism in promastigotes of Leishmania donovani

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1997 English Publisher:Springer Science and Business Media LLCJournal:Molecular and Cellular Biochemistry, volume 171, pages 65-68 (issn: 0300-8177, eissn: 1573-4919,

Authors: Vikas C. Pandey; Guru Dutt Sharma; Anil K. Rastogi; Kamal K. Kamboj; Pratima Srivastava;

doi: 10.1023/a:1006830113376

pmid: 9201697

Heme metabolism in promastigotes of Leishmania donovani

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Abstract

Promastigotes of Leishmania donovani (Dd-8 strain) showed presence of important key enzymes of heme synthesizing (delta-aminolevulinic acid synthase and ferrochelatase) and degrading (heme oxygenase and biliverdin reductase) systems, classical leishmanicidal drugs viz allopurinol, amphotericin B, pentamidine and CDRI compound 93/202 inhibited the heme oxygenase activity of the parasite, whereas, delta-aminolevulinic acid synthase activity practically remained unaffected. The Km, Vmax and pH values of heme oxygenase of promastigotes were found to be 1666 microM hemin, 625 nmol of bilirubin formed h-1 mg protein-1 and 7.5 respectively. The findings suggest the presence and importance of heme metabolism in the de novo synthesis of different hemoproteins of the Leishmania parasite as well as the detoxification and its defence against biological insults.

Related Organizations

Central Drug Research Institute
India

Keywords

Oxidoreductases Acting on CH-CH Group Donors, Heme Oxygenase (Decyclizing), Protozoan Proteins, Animals, Hydrogen-Ion Concentration, Oxidoreductases, Ferrochelatase, 5-Aminolevulinate Synthetase, Leishmania donovani

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