Identification of Caerulomycin A Gene Cluster Implicates a Tailoring Amidohydrolase
Copyright policy )Identification of Caerulomycin A Gene Cluster Implicates a Tailoring Amidohydrolase
The biosynthetic gene cluster for caerulomycin A (1) was cloned and characterized from the marine actinomycete Actinoalloteichus cyanogriseus WH1-2216-6, which revealed an unusual hybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) system. The crmL disruption mutant accumulated caerulomycin L (2) with an extended L-leucine at C-7, implicating an amidohydrolase activity for CrmL. The leucine-removing activity was confirmed for crude CrmL enzymes. Heterologous expression of the 1 gene cluster led to 1 production in Streptomyces coelicolor.
- Chinese Academy of Sciences China (People's Republic of)
- Ocean University of China China (People's Republic of)
- South China Sea Institute Of Oceanology China (People's Republic of)
Molecular Structure, Genes, Bacterial, Leucine, Pyridines, Multigene Family, Actinomycetales, Amino Acid Sequence, Peptide Synthases, Amidohydrolases
Molecular Structure, Genes, Bacterial, Leucine, Pyridines, Multigene Family, Actinomycetales, Amino Acid Sequence, Peptide Synthases, Amidohydrolases
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