From the leaves of Stenochlaena palustris five new O-acylated flavonol glycosides, stenopalustrosides A-E (1-5), have been isolated along with five known compounds, kaempferol 3-O-(3' '-O-E-p-coumaroyl)-(6' '-O-E-feruloyl)-beta-D-glucopyranoside (6), kaempferol 3-O-(3' ',6' '-di-O-E-p-coumaroyl)-beta-D-glucopyranoside (7), kaempferol 3-O-(3' '-O-E-p-coumaroyl)-beta-D-glucopyranoside (8), kaempferol 3-O-(6' '-O-E-p-coumaroyl)-beta-D-glucopyranoside (9); and kaempferol 3-O-beta-D-glucopyranoside (10). The structures of the isolates were elucidated by spectroscopic methods, mainly 1D and 2D NMR. Compounds 1-4 showed significant antibacterial activities against Gram-positive strains. The structural difference between the isolated antibacterial and nonantibacterial compounds is discussed.