Methyl isothiocyanate (MITC) is used as soil fumigant for several vegetable diseases and as antifermentative agent in wine. A new headspace solid phase microextraction (HS-SPME) method for analysis of MITC in wine is here described. This method is fast, inexpensive and does not require any solvent. Moreover, it shows a low minimum detectable limit and a wide linearity range. The quantification of MITC is performed by the standard additions method and does not require the use of internal standard, as in other methods. Keywords: Wine analysis; antifermentative agents; methyl isothiocyanate; HS-SPME