Hyperproduction of araC protein from Escherichia coli
Copyright policy )Hyperproduction of araC protein from Escherichia coli
Hypersynthesis of araC protein from Escherichia coli has been accomplished. The araC gene was cloned on plasmid pBR322, and some of the noncoding DNA preceding the araC gene was removed by exonuclease digestion. Finally, a DNA fragment containing the lac promoter and ribosome binding site was placed in front the araC gene. By these means the level of araC protein was increased about 5000-fold above the levels found in wild-type cells. This level of protein permits straight forward purification of sizeable quantities of araC protein.
Escherichia coli Proteins, AraC Transcription Factor, Repressor Proteins, Bacterial Proteins, Lac Operon, Genes, Bacterial, Escherichia coli, Cloning, Molecular, Plasmids, Transcription Factors
Escherichia coli Proteins, AraC Transcription Factor, Repressor Proteins, Bacterial Proteins, Lac Operon, Genes, Bacterial, Escherichia coli, Cloning, Molecular, Plasmids, Transcription Factors
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