Bacteria from the genus Clostridia, are responsible for such diseases as gas gangrene, wound infections, botulism, tetanus, pseudomembranous colitis, septicemia and nosocomial diarrhea. These anaerobic spores pose a special threat to man because they are ubiquitous, very difficult to kill, and tenaceously grow when conditions are right. This paper examines the process by which Clostridial endospores (specifically C.sporogenes ATCC 3584 and C. difficile ATCC 9689 & 43594) attach to a substrate and germinate, beginning the infective process.C.sporogenes and C.difficile endospores were anerobically incubated at 35 C on CDC blood agar (BCDC) and Trypticase Soy agar(TSA) with 0.5% glucose (BD, Cockeysville, MD). Control and experimental plates were identically streaked with spore suspension using a 1 microliter loop. The control plates were allowed to incubate and grow undisturbed for the duration of the experiment. Attachment experiments were done by streaking plates with either C.sporogenes or C. difficile spore suspensions (initially used full strength, but subsequently diluted for colony counting), incubating these spores in the anaerobic hood (Forma Scientific, Marietta, Ohio) on pre-reduced plates for 27 min, 1 hr 39 min, 2 hr 30 min, 2hr 39 min and 6 hrs.