Ceratocystis s.l. includes Ceratocystis s.s., Ophiostoma and Ceratocystiopsis . These genera are distinguished by their anamorphs, ascospore morphology and sensitivity to cycloheximide. Ceratocystis autographa is unusual in that it reportedly has both Ophiostoma and Ceratocystis anamorphs. The aim of this study was to clarify confusion relating to the anamorphs of C. autographa and thus its generic placement. Light and scanning electron microscopy confirmed the presence of two distinct anamorphs in the fungus. One was Sporothrix -like and very rare while the other was Chalara -like. Light and fluorescence microscopy, as well as scanning (SEM) and transmission (TEM) electron microscopy of the Chalara -like anamorph revealed cylindrical conidia with only a basal delimiting septum. TEM of this state showed vesicles at the apex of the conidiogenous cell, indicating apical wall building. This differs from other species of Chalara where conidia have apical and basal delimiting septa and develop through ring wall building. Furthermore, C. autographa was tolerant to cycloheximide and is, therefore, more closely related to Ophiostoma than Ceratiocystis . Results of this study suggest that the Chalara -like anamorph belongs to a genus other than Chalara and is a probable result of convergent evolution in a fungus adapted to insect dispersal.