Potentiation of Developing Synapses by Postsynaptic Release of Neurotrophin-4
Copyright policy )Potentiation of Developing Synapses by Postsynaptic Release of Neurotrophin-4
The hypothesis that synaptic functions can be regulated by neurotrophins secreted from the postsynaptic cell was examined in Xenopus nerve-muscle cultures. Neuromuscular synapses formed on myocytes overexpressing neurotrophin-4 (M+ synapses) exhibited a higher level of spontaneous synaptic activity and enhanced evoked synaptic transmission as compared to those formed on normal control myocytes (M- synapses). The NT-4 effects involve a potentiation of presynaptic transmitter secretion as well as a lengthening of the mean burst duration of postsynaptic low conductance acetylcholine channels. Repetitive stimulation of either the presynaptic neuron or the postsynaptic myocyte led to a potentiation of synaptic transmission at M+ synapses. All potentiation effects of NT-4 overexpression were abolished by the extracellular presence of TrkB-IgG but not by the presence of TrkA-IgG, indicating that postsynaptic secretion of NT-4 was responsible for the synaptic modification.
- University of California, San Diego United States
Male, Neurons, Embryo, Nonmammalian, Patch-Clamp Techniques, Neuroscience(all), Muscles, Green Fluorescent Proteins, Neuromuscular Junction, Receptor Protein-Tyrosine Kinases, Fertilization in Vitro, Electric Stimulation, Luminescent Proteins, Proto-Oncogene Proteins, Animals, Female, Nerve Growth Factors, RNA, Messenger, Receptor, trkA, Evoked Potentials, Receptor, Ciliary Neurotrophic Factor, Cells, Cultured
Male, Neurons, Embryo, Nonmammalian, Patch-Clamp Techniques, Neuroscience(all), Muscles, Green Fluorescent Proteins, Neuromuscular Junction, Receptor Protein-Tyrosine Kinases, Fertilization in Vitro, Electric Stimulation, Luminescent Proteins, Proto-Oncogene Proteins, Animals, Female, Nerve Growth Factors, RNA, Messenger, Receptor, trkA, Evoked Potentials, Receptor, Ciliary Neurotrophic Factor, Cells, Cultured
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