
pmid: 2904197
A molecular probe for the PT-operon of B. pertussis hybridized with 4.7 Kb EcoRI-fragments of chromosomal DNAs of B. pertussis strain 475 phase I, phase IV, B. parapertussis strains 504 and 17903, B. bronchiseptica strain 214, B. parapertussis strain 17903-convertant of B. pertussis phage 134 but not with phage 134 DNA under stringent conditions of DNA-DNA hybridization. This fact indicates the presence of PT-genes in all Bordetella species. Since there is no production of PT in B. parapertussis and B. bronchiseptica, a presence of regulatory mutations in the PT-operon or absence of the functionally active vir-gene product in these species is suggested.
DNA, Bacterial, Bordetella, Nucleic Acid Hybridization, DNA Restriction Enzymes, Bordetella pertussis, Deoxyribonuclease EcoRI, Blotting, Southern, Pertussis Toxin, Genes, Bacterial, Virulence Factors, Bordetella, Cloning, Molecular, DNA Probes, Deoxyribonucleases, Type II Site-Specific, Plasmids
DNA, Bacterial, Bordetella, Nucleic Acid Hybridization, DNA Restriction Enzymes, Bordetella pertussis, Deoxyribonuclease EcoRI, Blotting, Southern, Pertussis Toxin, Genes, Bacterial, Virulence Factors, Bordetella, Cloning, Molecular, DNA Probes, Deoxyribonucleases, Type II Site-Specific, Plasmids
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