Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA Silencing Pathways
Copyright policy )Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA Silencing Pathways
The RNase III enzyme Dicer processes RNA into siRNAs and miRNAs, which direct a RNA-induced silencing complex (RISC) to cleave mRNA or block its translation (RNAi). We have characterized mutations in the Drosophila dicer-1 and dicer-2 genes. Mutation in dicer-1 blocks processing of miRNA precursors, whereas dicer-2 mutants are defective for processing siRNA precursors. It has been recently found that Drosophila Dicer-1 and Dicer-2 are also components of siRNA-dependent RISC (siRISC). We find that Dicer-1 and Dicer-2 are required for siRNA-directed mRNA cleavage, though the RNase III activity of Dicer-2 is not required. Dicer-1 and Dicer-2 facilitate distinct steps in the assembly of siRISC. However, Dicer-1 but not Dicer-2 is essential for miRISC-directed translation repression. Thus, siRISCs and miRISCs are different with respect to Dicers in Drosophila.
- Northwestern University United States
- Northwestern State University United States
Male, Ribonuclease III, Sequence Homology, Amino Acid, Biochemistry, Genetics and Molecular Biology(all), Molecular Sequence Data, Protein Structure, Tertiary, MicroRNAs, Drosophila melanogaster, Protein Biosynthesis, Mutation, Animals, Drosophila Proteins, RNA-Induced Silencing Complex, Female, RNA Interference, Eye Abnormalities, RNA, Messenger, RNA, Small Interfering, RNA Helicases
Male, Ribonuclease III, Sequence Homology, Amino Acid, Biochemistry, Genetics and Molecular Biology(all), Molecular Sequence Data, Protein Structure, Tertiary, MicroRNAs, Drosophila melanogaster, Protein Biosynthesis, Mutation, Animals, Drosophila Proteins, RNA-Induced Silencing Complex, Female, RNA Interference, Eye Abnormalities, RNA, Messenger, RNA, Small Interfering, RNA Helicases
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