Publisher Summary This chapter emphasizes on the two generally useful techniques of mammalian cell culture: monolayer cultures grown adherent to a glass surface and suspension cultures agitated by a magnetic "spinner". Mammalian cells in culture may be considered as fastidious microorganisms with a relatively long generation time. This point of view serves to indicate the nature of the technical problems involved in cell culture. It is desirable to minimize the likelihood of airborne contamination of cultures by reducing the amount of dust in the laboratory. Useful measures to this end include filtering incoming air and oiling the bench tops at regular intervals. The "spinner" culture technique requires an incubator in which magnetic stirring bases may be installed. Monolayer cultures are conveniently grown in the T-series of Pyrex flasks which provide effective surfaces of 9 to 60 cm. One-liter Blake bottles are conveniently used for larger cultures. Inexpensive soft-glass prescription bottles or milk dilution bottles are satisfactory substitutes for the flasks in many instances. In the spinner technique for suspension cultures, the cells are grown in bottles fitted with a Teflon-covered magnetic bar suspended from a swivel by a stainless-steel wire.