Human embryonic stem cells hold great promise in furthering our treatment of disease and increasing our understanding of early development. This chapter describes protocols for the derivation and maintenance of human embryonic stem cells. In addition, it summarizes briefly several alternative methods for the culture of human embryonic stem cells. Thus, this chapter provides a good starting point for researchers interested in harnessing the potential of human embryonic stem cells.

Related Organizations

Harvard University
United States

Keywords

Cell Culture Techniques, Cell Separation, Fibroblasts, Embryo, Mammalian, Cell Line, Culture Media, Mice, Blastocyst, Animals, Humans, Trypsin, Collagenases, Cell Division, Embryonic Stem Cells

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