<script type="text/javascript">
<!--
document.write('<div id="oa_widget"></div>');
document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=undefined&type=result"></script>');
-->
</script>

COPY SCRIPT

For further information contact us at helpdesk@openaire.eu

Isolation and identification of the cyanotoxin cylindrospermopsin and deoxy-cylindrospermopsin from a Thailand strain of Cylindrospermopsis raciborskii (Cyanobacteria)

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2001 Australia English Publisher:Elsevier BVJournal:Toxicon, volume 39, pages 973-980 (issn: 0041-0101,

Authors: Li, R.; Carmichael, W. W.; Brittain, S.; Eaglesham, G. K.; Shaw, G. R.; Mahakhant, A.; Noparatnaraporn, N.; +3 Authors

doi: 10.1016/s0041-0101(00)00236-1

pmid: 11223086

handle: 10072/58361

Isolation and identification of the cyanotoxin cylindrospermopsin and deoxy-cylindrospermopsin from a Thailand strain of Cylindrospermopsis raciborskii (Cyanobacteria)

- Summary
- Subjects
- Related research
  (9)
- Metrics

Abstract

A strain of Cylindrospermopsis (Cyanobacteria) isolated from a fishpond in Thailand was examined for its taxonomy based upon morphology and 16S rRNA gene sequence. It was also examined for production of the hepatotoxic cyanotoxin called cylindrospermopsin (CYN) and deoxycylindrospermopsin (deoxy-CYN). The strain (CY-Thai) was identified as C. raciborskii (Woloszynska) Seenaya and Subba Raju based upon morphological examination which was confirmed by 16S rRNA gene sequences and phylogenetic comparisons based upon its 16S rRNA gene. The alkaloid heptatotoxin CYN was confirmed using mouse bioassay, HPLC and HPLC-MS/MS while deoxy-CYN was confirmed using HPLC-MS/MS. The mouse bioassay gave a minimum lethal dose at 250mg dry weight cells/kg body weight within 24h and 125mg/kg at 72h, with signs of poisoning the same as in literature reports for CYN. HPLC chromatographic comparison of the CY-Thai toxin with standard CYN gave the same retention time and an absorbance maximum at 262nm. HPLC-MS/MS confirmed the presence of CYN (M+H 416) and deoxy-CYN (M+H 400). The CYN content in strain CY-Thai was estimated at 1.02mg/g and approximately 1/10 of this amount for deoxy-CYN. This is the first report from Asia of a CYN, deoxy-CYN producing Cylindrospermopsis raciborskii.

Country

Australia

Related Organizations

View all View all

Keywords

572, Bacterial Toxins, Woloszynska, Toxicology, Cyanobacteria, Mass Spectrometry, Mice, C1, Alkaloids, Cyanophyceae, Deoxy-cylindrospermopsin, Animals, Pharmacology & Pharmacy, 321299 Public Health and Health Services not elsewhere classified, Uracil, Chromatography, High Pressure Liquid, Toxicity, Cyanobacteria Toxins, 730210 Environmental health, Reverse Transcriptase Polymerase Chain Reaction, Palm-island, Pharmacology and Pharmaceutical Sciences, Thailand, Death, Lakes, Cyanotoxin, Cylindrospermopsin, RNA, Ribosomal, Queensland, Toxin, 1115 Pharmacology and Pharmaceutical Sciences, Cylindrospermopsis

9 Research products, page of 1

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	147
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%