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Analysis and significance of anti-nuclear antibodies in dogs

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1997Publisher:Elsevier BVJournal:Research in Veterinary Science, volume 62, pages 83-84 (issn: 0034-5288,

Authors: A. S. M. Bari; D. F. Kelly; Stuart D. Carter; S. C. Bell; David L.H. Bennett; Dez Hughes;

doi: 10.1016/s0034-5288(97)90187-9

pmid: 9160432

Analysis and significance of anti-nuclear antibodies in dogs

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Abstract

Assays for detecting and measuring antinuclear antibodies (ANA) in dogs were compared. They included the indirect immunofluorescence test, using rat liver as substrate, and ELISAs for three nuclear antigens: double-stranded DNA, single-stranded DNA, and histone. There was no correlation between the ANA titre and antibodies to the three nuclear antigens. Analysis of ANA in different arthropathies showed no specific disease association. HEP-2 cells showed no fluorescence reaction with either ANA-positive or ANA-negative dog sera. Western blotting produced too complex a pattern to identify specific antigens. The antigens that reacted with ANA in dogs were not identified; there is either a broad range of reactivities or non-specific binding of immunoglobulins.

Related Organizations

University of Liverpool
United Kingdom

Keywords

Blotting, Western, DNA, Single-Stranded, Enzyme-Linked Immunosorbent Assay, DNA, Rats, Histones, Dogs, Antibodies, Antinuclear, Animals, Fluorescent Antibody Technique, Indirect

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