Analysis of chromatin in fission yeast

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2004 English Publisher:Elsevier BVJournal:Methods, volume 33, pages 252-259 (issn: 1046-2023,

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Authors: Alison, Pidoux; Barbara, Mellone; Robin, Allshire;

doi: 10.1016/j.ymeth.2003.11.021 , 10.1016/s1046-2023(03)00320-7

pmid: 15157893

Analysis of chromatin in fission yeast

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Abstract

The use of fission yeast as a model system for studies of chromosome biology has contributed to several key advances in the last few years. The structure of its large complex centromeres and composition of its transcriptionally silent heterochromatin resemble those of metazoa. The application of chromatin immunoprecipitation to fission yeast has been instrumental in these advances and we describe an improved version of this technique in detail. In addition, we describe several other techniques, which are useful in the analysis of chromatin in fission yeast.

Related Organizations

University of Edinburgh
United Kingdom
Wellcome Centre for Cell Biology
United Kingdom

Keywords

Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Chromatin

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