Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta
Copyright policy )Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta
Plant viruses have evolved to undermine the RNA silencing pathway by expressing suppressor protein(s) that interfere with one or more key components of this antiviral defense. Here we show that the recently identified RNA silencing suppressor (RSS) of lettuce necrotic yellows virus (LNYV), phosphoprotein P, binds to RNA silencing machinery proteins AGO1, AGO2, AGO4, RDR6 and SGS3 in protein-protein interaction assays when transiently expressed. In planta, we demonstrate that LNYV P inhibits miRNA-guided AGO1 cleavage and translational repression, and RDR6/SGS3-dependent amplification of silencing. Analysis of LNYV P deletion mutants identified a C-terminal protein domain essential for both local RNA silencing suppression and interaction with AGO1, AGO2, AGO4, RDR6 and SGS3. In contrast to other viral RSS known to disrupt AGO activity, LNYV P sequence does not contain any recognizable GW/WG or F-box motifs. This suggests that LNYV P may represent a new class of AGO binding proteins.
- University of Queensland Australia
- University of Queensland Australia
- University of Queensland Australia
Nicotiana, Protein-protein interactions, Agroinfiltration, Protein–protein interactions, Argonaute proteins, Lettuce necrotic yellows cytorhabdovirus, Viral Proteins, Phosphoprotein, Virology, Argonaute Proteins, 2406 Virology, RNA, Viral, RNA silencing amplification, RNA Interference, Rhabdoviridae, RNA silencing suppression, Plant Diseases, Plant Proteins
Nicotiana, Protein-protein interactions, Agroinfiltration, Protein–protein interactions, Argonaute proteins, Lettuce necrotic yellows cytorhabdovirus, Viral Proteins, Phosphoprotein, Virology, Argonaute Proteins, 2406 Virology, RNA, Viral, RNA silencing amplification, RNA Interference, Rhabdoviridae, RNA silencing suppression, Plant Diseases, Plant Proteins
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