Replication of Lettuce chlorosis virus (LCV), a crinivirus in the family Closteroviridae, is accompanied by the production of LCV RNA 1-derived novel RNAs
Copyright policy )Replication of Lettuce chlorosis virus (LCV), a crinivirus in the family Closteroviridae, is accompanied by the production of LCV RNA 1-derived novel RNAs
Cloned infectious complementary DNAs of the bipartite genomic RNAs of Lettuce chlorosis virus (LCV) were constructed. Inoculation of tobacco protoplasts with the in vitro produced RNAs 1 and 2 transcripts, or with RNA 1 transcript alone, resulted in viral replication accompanied by the production of novel LCV RNA 1-derived RNAs. They included the abundantly accumulating LM-LCVR1-1 (~0.38 kb) and LM-LCVR1-2 (~0.3 kb), and the lowly accumulating HM-LCVR1-1 (~8.0 kb) and HM-LCVR1-2 (~6.6 kb), all of which reacted with riboprobes specific to the 5' end of RNA 1 in Northern blot analysis. LM-LCVR1-1 and HM-LCVR1-2 accumulated as positive-stranded RNAs that lacked complementary negative strands, while HM-LCVR1-1 and LM-LCVR1-2 accumulated in both polarities. Additional Northern blot, reverse transcription-polymerase chain reaction, cloning, and sequence analyses revealed LM-LCVR1-2 to be an authentic RNA 1-derived defective (D)RNA, suggesting that its synthesis and maintenance are supported in trans by an RNA 1 encoded replication machinery.
- University of California System United States
- University of California, San Francisco United States
- University of California, Riverside United States
Defective RNA, Nicotiana, Crinivirus, DNA, Complementary, Base Sequence, Transcription, Genetic, 5′-terminal RNA, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, RNA, Subgenomic RNA, Genome, Viral, Blotting, Northern, Virus Replication, Cloned infectious cDNAs, Virology, RNA, Viral, Lettuce infectious yellows virus, Cloning, Molecular, Lactuca, Plant Diseases
Defective RNA, Nicotiana, Crinivirus, DNA, Complementary, Base Sequence, Transcription, Genetic, 5′-terminal RNA, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, RNA, Subgenomic RNA, Genome, Viral, Blotting, Northern, Virus Replication, Cloned infectious cDNAs, Virology, RNA, Viral, Lettuce infectious yellows virus, Cloning, Molecular, Lactuca, Plant Diseases
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