Conductive Velcro fasteners are employed as a joining material for various types of connections due to their detachable properties, especially when an electrical connection is established. Due to microscopic actual contact spots between the fibers and hooks in the Velcro joints, a large electrical contact resistance is established that could cause heat and potentially short-circuit. In this study, the electrical contact resistance behaviors in the conductive Velcro are estimated according to the electrical load, frequency and load amplitude. The wear behavior due to these effects is investigated after long exposure to dynamic loading under a specific frequency at a low load amplitude.