Abstract The inclusion of multiple new markers, in particular 41 sequence specific markers, resulted in drastically improved version of a previously published F1-based genetic map of pineapple (Ananas comosus). The integration of the new version of this map with a more recently published F2-based map resulted in a map that assembles 741 loci: 739 DNA markers (25 SSR, 12 EST_SSR, 22 SCAR, 8 CAPS, 20 ISSR, 109 RAPD, and 543 AFLP), one isozyme (PGM) locus and the morphological trait ‘piping’, in 28 integrated linkage groups, spanning 2113 centimorgans (cM) and covering approximately 86% of the genome. Four small F1-based linkage groups and 5 small F2-based linkage groups assembling more than two markers, totalling 292 cM, remained not integrated. The present integrated genetic map is expected to be a helpful tool in genomic studies on pineapple and other Bromeliaceae genera and species.