Biocidal polymers were prepared by reacting uramil with polyacrylonitrile and polyethylacrylate, and also by co-polymerizing heterocyclic monomers based on uramil azo-dyes with tolylene-2,6-diisocyanate and toluene-2,4-diisocyanate. The prepared polymers were converted to their quaternary salts and N-halamine forms. Prepared N-halamine polymers were designed to contain multi-available positions for halogenation and quaternarization and to tune the stability of halogens attached to the polymers. Antimicrobial activity of the prepared polymers against examples of Gram-positive and Gram-negative bacteria was examined.