
ResumenIntroducciónLa esporotricosis es una infección causada por especies pertenecientes al complejo Sporothrix schenckii. Dependiendo de la especie, estos organismos pueden tener virulencia y formas clínicas diferentes.ObjetivoVerificar la virulencia de una cepa de Sporothrix globosa en un modelo murino usando 2 concentraciones de inóculo, en aplicación intraperitoneal o subcutánea.Materiales y métodosEstudio experimental no aleatorizado en murinos inoculados con una cepa de S. globosa (CBS 14.076M), por vía intraperitoneal y subcutánea, a 0,5 y 4 de concentración McFarland. Se utilizaron 18 roedores CF-1 (ISP, Santiago, Chile).ResultadosLa cepa estudiada no provocó enfermedad ni lesiones, todos los animales sobrevivieron, no hubo desarrollo de hongos en el cultivo de tejidos y el análisis histopatológico de órganos no mostró alteraciones sugerentes de infección.ConclusionesLa cepa de S. globosa estudiada no presentó virulencia en modelos murinos y no provocó enfermedad al ser inoculada a concentración McFarland 0,5 y 4, tanto por vía intraperitoneal como subcutánea.AbstractIntroductionThe sporothricosis disease is an infection caused by species included in Sporothrix schenkii complex.ObjectiveVerify the virulence of a strain of S. globosa using two different concentrations of inoculum by intraperitoneally and subcutaneously, into a mouse model.Materials and methodsNonrandomized pilot study, in murine inoculated with a strain of S. globosa (CBS 14.076M) by intraperitoneally and subcutaneously with inoculum concentrations of 0.5 and 4 McFarland. For this purpose 18 rodents CF-1 (ISP, Santiago, Chile) were used.ResultsThe studied strain did not induce illness or injury on animals, they all survived and neither the tissue culture nor the histopathological analysis showed fungal growth or suggestive infection by organ abnormalities.ConclusionsThe S. globosa strain did not present any virulence enough to cause disease at 0.5 and 4.0 McFarland concentration inoculum when inoculated in both intraperitoneally and subcutaneously, in murine models.
Microbiology (medical), Esporotricosis, Patogenicidad, Sporothrix globosa, Pathogenicity, Microbiology, Sporotrichosis
Microbiology (medical), Esporotricosis, Patogenicidad, Sporothrix globosa, Pathogenicity, Microbiology, Sporotrichosis
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