AbstractGas Metal Arc Welding (GMAW) process is leading in the development in arc welding process which is higher productivity and good in quality. In this study, the effects of different parameters on welding penetration, microstructural and hardness measurement in mild steel that having the 6mm thickness of base metal by using the robotic gas metal arc welding are investigated. The variables that choose in this study are arc voltage, welding current and welding speed. The arc voltage and welding current were chosen as 22, 26 and 30V and 90, 150 and 210 A respectively. The welding speed was chosen as 20, 40 and 60cm/min. The penetration, microstructure and hardness were measured for each specimen after the welding process and the effect of it was studied. As a result, it obvious that increasing the parameters value of welding current increased the value of depth of penetration. Other than that, arc voltage and welding speed is another factor that influenced the value of depth of penetration. The microstructure shown the different grain boundaries of each parameters that affected of the welding parameters.