AbstractAn impedance based electrochemical biosensor was successfully printed using a rotogravure printing technique. The biosensor was fabricated on a flexible poly ethylene terephthalate (PET) substrate and silver (Ag) nano particle based ink was used as metallization for the printed interdigitated electrodes (IDE's), with dimensions of 200μm width and spacing. The response of the printed device towards different bio/chemical species such as cadmium sulphide (CdS), lead sulphide (PbS), D-proline and mouse IgG were measured using electrochemical impedance spectroscopy (EIS). The response of the printed biosensors towards these bio/chemicals demonstrated detection capabilities as low as picomolar levels.