Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD)

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 2005 Malaysia English Publisher:Elsevier BVJournal:Process Biochemistry, volume 40, pages 753-758 (issn: 1359-5113,

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Authors: Ibrahim, Hanan Moawia; Wan Yusoff, Wan Mokhtar; Abdul Hamid, Aidil; Md. Illias, Rosli; Hassan, Othman; Omar, Othman;

doi: 10.1016/j.procbio.2004.01.042

Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD)

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Abstract

Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9 U/ml) was determined. A 25 Central Composite Design (CCD; half fraction) has been chosen to elucidate the combined effect of five process variables; tapioca starch, peptone, yeast extract, magnesium sulphate and potassium phosphate concentration. Concentrations of 4% tapioca starch, 2% peptone, 0.04% magnesium sulphate and 1% sodium carbonate have been shown to be optimum for the production of CGTase.

Country

Malaysia

Related Organizations

National University of Malaysia
Malaysia
UNIVERSITI TEKNOLOGI MARA (UTM)
Malaysia
Universiti Malaysia Terengganu
Malaysia

Keywords

T Technology (General)

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