The role played by certain factors, physical barriers and chitinase enzyme, associated with blister blight resistance in tea was investigated. The blister blight resistance of the tea clone SA-6 was due to higher amounts of epicuticular wax and increased thickness of cuticle/epidermal layer, functioning as physical barriers to hyphal penetration of Exobasidium vexans. Higher quantum of chitinase in intercellular spaces of palisade tissues was observed in the resistant clone SA-6 through immunolocalization study. Both chitinase assay as well as western blotting studies confirmed that the constitutive level of chitinase expression was higher in the resistant clone when compared with the susceptible tea clone.