Transplacental transfer of melamine
Copyright policy )Transplacental transfer of melamine
To characterize transplacental transfer of melamine and related mechanisms as well as toxicity using human placental perfusion and cultured cells.Transfer and toxicity were analyzed in 4-h perfusions with 10 μM or 1 mM melamine, or 10 μM melamine with 10 nM cyanuric acid (CYA). Efflux transporters were studied in accumulation assay and toxicity in BeWo cells by MTT assay.Of added melamine 34-45% was transferred to fetal circulation and CYA made no difference. Histology, hCG production, and PLAP activity indicated functionality of placental tissue with no grave toxicity. Highest concentration of melamine used (2 mM) with CYA and long treatment time decreased viability of BeWo cells. Inhibitors of ABCB1, ABCG2, ABCC2 did not affect the accumulation of melamine in cells.Melamine goes through human term placenta with no contribution of efflux transporters. Toxicity of melamine is low in placental tissue and BeWo cells.
- University of Hong Kong (香港大學) China (People's Republic of)
- Oulu University Hospital Finland
- University of Eastern Finland Finland
- University of Hong Kong China (People's Republic of)
- University of Oulu Finland
ATP-Binding Cassette, Sub-Family C Proteins, ATP Binding Cassette Transporter, Subfamily B, Cell Survival - drug effects, Placenta - blood supply - cytology - drug effects - physiology, Cell Survival, Placenta, Perfusion - methods, P-Glycoprotein - antagonists & inhibitors, In Vitro Techniques, Chorionic Gonadotropin, Chorionic Gonadotropin - secretion, Cell Line, ATP-Binding Cassette Transporters - antagonists & inhibitors, Pregnancy, Triazines - metabolism - pharmacology - toxicity, Membrane Transport Modulators, Resins, Synthetic - metabolism - toxicity, Synthetic - metabolism - toxicity, ATP Binding Cassette Transporter, Subfamily G, Member 2, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 1, Membrane Transport Modulators - pharmacology, Maternal-Fetal Exchange, Maternal-Fetal Exchange - drug effects, Neoplasm Proteins - antagonists & inhibitors, Pregnancy Proteins - antagonists & inhibitors - metabolism - secretion, Biological Transport, Trophoblasts - cytology - drug effects - metabolism, Biological Transport - drug effects, Multidrug Resistance-Associated Protein 2, Neoplasm Proteins, Perfusion, Kinetics, Multidrug Resistance-Associated Proteins - antagonists & inhibitors, ATP-Binding Cassette Transporters, Female, Resins
ATP-Binding Cassette, Sub-Family C Proteins, ATP Binding Cassette Transporter, Subfamily B, Cell Survival - drug effects, Placenta - blood supply - cytology - drug effects - physiology, Cell Survival, Placenta, Perfusion - methods, P-Glycoprotein - antagonists & inhibitors, In Vitro Techniques, Chorionic Gonadotropin, Chorionic Gonadotropin - secretion, Cell Line, ATP-Binding Cassette Transporters - antagonists & inhibitors, Pregnancy, Triazines - metabolism - pharmacology - toxicity, Membrane Transport Modulators, Resins, Synthetic - metabolism - toxicity, Synthetic - metabolism - toxicity, ATP Binding Cassette Transporter, Subfamily G, Member 2, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 1, Membrane Transport Modulators - pharmacology, Maternal-Fetal Exchange, Maternal-Fetal Exchange - drug effects, Neoplasm Proteins - antagonists & inhibitors, Pregnancy Proteins - antagonists & inhibitors - metabolism - secretion, Biological Transport, Trophoblasts - cytology - drug effects - metabolism, Biological Transport - drug effects, Multidrug Resistance-Associated Protein 2, Neoplasm Proteins, Perfusion, Kinetics, Multidrug Resistance-Associated Proteins - antagonists & inhibitors, ATP-Binding Cassette Transporters, Female, Resins
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).20 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!