Abstract Further chemical investigation of the butanol fraction of Olax mannii Oliv. (Olacaceae) methanol leaf extract led to the isolation of two new kaempferol triglycosides namely: kaempferol 3-O-[α- l -rhamnopyranosyl (1 → 2) α- l -arabinofuranoside]-7-O-α- l -rhamnopyranoside, (1) and kaempferol 3-O-[α- l -apiofuranosyl (1 → 2) β-D- galactopyranoside]-7-O-α- l -rhamnopyranoside (2). The structures of the isolated compounds were determined by detailed analysis of their one- and two-dimensional NMR and high-resolution mass spectra. The compounds were screened for antimicrobial activity against strains of bacteria and fungi.