Glycosylation of daidzein by the Eucalyptus cell cultures
Copyright policy )Glycosylation of daidzein by the Eucalyptus cell cultures
The sequential glycosylation of a soybean isoflavone, daidzein, with cultured suspension cells of Eucalyptus perriniana and cyclodextrin glucanotransferase was studied. Daidzein was converted into two glycosylation products, daidzein 7-O-beta-D-glucopyranoside (39%) and daidzein 7-O-[6-O-(beta-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-gentiobioside, 6%), by cultured E. perriniana cells. Further glycosylation of daidzein 7-O-beta-glucoside with cyclodextrin glucanotransferase gave daidzein 7-O-[4-O-(alpha-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-maltoside, 26%), daidzein 7-O-beta-maltotrioside (15%), and daidzein 7-O-beta-maltotetraoside (7%).
Cyclodextrins, Eucalyptus, Kinetics, Glycosylation, Glucosides, Phenols, Glucosyltransferases, Isoflavones, Cells, Cultured
Cyclodextrins, Eucalyptus, Kinetics, Glycosylation, Glucosides, Phenols, Glucosyltransferases, Isoflavones, Cells, Cultured
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