Characterization of Plasmodium berghei glutathione synthetase
Copyright policy )Characterization of Plasmodium berghei glutathione synthetase
Plasmodium berghei contained 0.454±0.031 U/mg of glutathione synthetyase (GS). GS was purified using solid ammonium sulfate and Sephadex G-200 from P. berghei infected mouse erythrocytes. SDS-PAGE showed purified GS as a single band protein of 70 kDa and its Km for γ-glutamylcysteine, glycine and ATP being 0.33 mM, 8.3 mM and 0.43 mM respectively with noncompetitive inhibition by GSH. The malaria parasite enzyme was optimally active at 37°C and pH 8.0-8.5. Heavy metals significantly inhibited parasite GS activity.
Erythrocytes, Plasmodium berghei, Glycine, Temperature, Dipeptides, Hydrogen-Ion Concentration, Glutathione, Glutathione Synthase, Malaria, Molecular Weight, Kinetics, Mice, Adenosine Triphosphate, Metals, Heavy, Animals, Humans, Subcellular Fractions
Erythrocytes, Plasmodium berghei, Glycine, Temperature, Dipeptides, Hydrogen-Ion Concentration, Glutathione, Glutathione Synthase, Malaria, Molecular Weight, Kinetics, Mice, Adenosine Triphosphate, Metals, Heavy, Animals, Humans, Subcellular Fractions
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).6 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!