Infantile esotropia (manifesting from birth to 6 months) is a common type of strabismus, accounting for 28% to 54% of all esotropias and with an incidence of 1% of the general population. The purpose of this cohort study was to evaluate risk factors for infantile esotropia. Such information may aid in early intervention to prevent manifestation of infantile esotropia.A retrospective chart review of 5,347 records (October 1, 1993, to September 30, 2003) of birth mothers and infants at the W.W. Hastings Indian Health Science Hospital in Tahlequah, Oklahoma, was performed to identify children with varying degrees of Native American blood who had infantile esotropia. A nonstrabismic birth cohort control group was also identified. Twenty-three medical records indicating a diagnosis of infantile esotropia that were complete enough to be used in analysis were identified. Normal infants were compared with infants with esotropia.Infantile esotropia in this preliminary study was associated with 24 factors including prematurity, family ocular history, cardiovascular disease, systemic disease, pregnancy-associated hypertension and low birth weight (<2,500 g) among others.Prematurity, family history or secondary ocular history, perinatal or gestational complications, systemic disorders, use of supplemental oxygen as a neonate, use of systemic medications, and male sex were found to be significant risk factors for infantile esotropia. Our results provide additional evidence that might help facilitate early detection and intervention in cases in which these risk factors are identified.