Ceramides and sphingomyelinases in senile plaques
Copyright policy )Ceramides and sphingomyelinases in senile plaques
The senile plaque is a hallmark lesion of Alzheimer disease (AD). We compared, without a priori, the lipidome of the senile plaques and of the adjacent plaque-free neuropil. The analysis by liquid chromatography coupled with electrospray ionization mass spectrometry revealed that laser microdissected senile plaques were enriched in saturated ceramides Cer(d18:1/18:0) and Cer(d18:1/20:0) by 33 and 78% respectively with respect to the surrounding neuropil. This accumulation of ceramides was not explained by their affinity for Aβ deposits: no interaction between ceramide-liposomes and Aβ fibrils was observed in vitro by surface plasmon resonance and fluorescent ceramide-liposomes showed no affinity for the senile plaques in AD brain tissue. Accumulation of ceramides could be, at least partially, the result of a local production by acid and neutral sphingomyelinases that we found to be present in the corona of the senile plaques.
- University of Lyon System France
- Sorbonne University France
- University of Milano-Bicocca Italy
- ParisTech France
- French Institute of Health and Medical Research France
Male, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Senile plaques, Neurosciences. Biological psychiatry. Neuropsychiatry, Plaque, Amyloid, Ceramides, Mass Spectrometry, Apolipoproteins E, Alzheimer Disease, [CHIM] Chemical Sciences, Humans, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Aged, Aged, 80 and over, [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Amyloid beta-Peptides, Mass spectrometry, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Aβ peptide; Alzheimer disease; Ceramides; Laser microdissection; Lipidomics; Lipids; Mass spectrometry; Senile plaques; Sphingomyelinase; Aged; Aged, 80 and over; Alzheimer Disease; Amyloid beta-Peptides; Apolipoproteins E; Ceramides; Chromatography, Liquid; Female; Humans; Male; Mass Spectrometry; Microdissection; Middle Aged; Plaque, Amyloid; Sphingomyelin Phosphodiesterase; Surface Plasmon Resonance; Neurology, Middle Aged, Surface Plasmon Resonance, [SDV] Life Sciences [q-bio], Aβ peptide; Alzheimer disease; Ceramides; Laser microdissection; Lipidomics; Lipids; Mass spectrometry; Senile plaques; Sphingomyelinase;, Sphingomyelin Phosphodiesterase, Lipidomics, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Female, Alzheimer disease, Laser microdissection, Microdissection, RC321-571, Chromatography, Liquid
Male, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Senile plaques, Neurosciences. Biological psychiatry. Neuropsychiatry, Plaque, Amyloid, Ceramides, Mass Spectrometry, Apolipoproteins E, Alzheimer Disease, [CHIM] Chemical Sciences, Humans, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Aged, Aged, 80 and over, [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Amyloid beta-Peptides, Mass spectrometry, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Aβ peptide; Alzheimer disease; Ceramides; Laser microdissection; Lipidomics; Lipids; Mass spectrometry; Senile plaques; Sphingomyelinase; Aged; Aged, 80 and over; Alzheimer Disease; Amyloid beta-Peptides; Apolipoproteins E; Ceramides; Chromatography, Liquid; Female; Humans; Male; Mass Spectrometry; Microdissection; Middle Aged; Plaque, Amyloid; Sphingomyelin Phosphodiesterase; Surface Plasmon Resonance; Neurology, Middle Aged, Surface Plasmon Resonance, [SDV] Life Sciences [q-bio], Aβ peptide; Alzheimer disease; Ceramides; Laser microdissection; Lipidomics; Lipids; Mass spectrometry; Senile plaques; Sphingomyelinase;, Sphingomyelin Phosphodiesterase, Lipidomics, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Female, Alzheimer disease, Laser microdissection, Microdissection, RC321-571, Chromatography, Liquid
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