TAIL-seq: Genome-wide Determination of Poly(A) Tail Length and 3′ End Modifications
Copyright policy )TAIL-seq: Genome-wide Determination of Poly(A) Tail Length and 3′ End Modifications
Global investigation of the 3' extremity of mRNA (3'-terminome), despite its importance in gene regulation, has not been feasible due to technical challenges associated with homopolymeric sequences and relative paucity of mRNA. We here develop a method, TAIL-seq, to sequence the very end of mRNA molecules. TAIL-seq allows us to measure poly(A) tail length at the genomic scale. Median poly(A) length is 50-100 nt in HeLa and NIH 3T3 cells. Poly(A) length correlates with mRNA half-life, but not with translational efficiency. Surprisingly, we discover widespread uridylation and guanylation at the downstream of poly(A) tail. The U tails are generally attached to short poly(A) tails (40 nt), implicating their generic roles in mRNA stability control. TAIL-seq is a potent tool to dissect dynamic control of mRNA turnover and translational control, and to discover unforeseen features of RNA cleavage and tailing.
- Seoul National University Korea (Republic of)
- Institute for Basic Science Korea (Republic of)
Genome, Guanine, Base Sequence, Sequence Analysis, RNA, RNA Stability, Molecular Sequence Data, Cell Biology, Polyadenylation, Mice, MicroRNAs, Gene Expression Regulation, NIH 3T3 Cells, Animals, Humans, Molecular Biology, 3' Untranslated Regions, Uridine, Half-Life, HeLa Cells, Signal Transduction
Genome, Guanine, Base Sequence, Sequence Analysis, RNA, RNA Stability, Molecular Sequence Data, Cell Biology, Polyadenylation, Mice, MicroRNAs, Gene Expression Regulation, NIH 3T3 Cells, Animals, Humans, Molecular Biology, 3' Untranslated Regions, Uridine, Half-Life, HeLa Cells, Signal Transduction
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