The Nuclear-Retained Noncoding RNA MALAT1 Regulates Alternative Splicing by Modulating SR Splicing Factor Phosphorylation
Copyright policy )The Nuclear-Retained Noncoding RNA MALAT1 Regulates Alternative Splicing by Modulating SR Splicing Factor Phosphorylation
Alternative splicing (AS) of pre-mRNA is utilized by higher eukaryotes to achieve increased transcriptome and proteomic complexity. The serine/arginine (SR) splicing factors regulate tissue- or cell-type-specific AS in a concentration- and phosphorylation-dependent manner. However, the mechanisms that modulate the cellular levels of active SR proteins remain to be elucidated. In the present study, we provide evidence for a role for the long nuclear-retained regulatory RNA (nrRNA), MALAT1 in AS regulation. MALAT1 interacts with SR proteins and influences the distribution of these and other splicing factors in nuclear speckle domains. Depletion of MALAT1 or overexpression of an SR protein changes the AS of a similar set of endogenous pre-mRNAs. Furthermore, MALAT1 regulates cellular levels of phosphorylated forms of SR proteins. Taken together, our results suggest that MALAT1 regulates AS by modulating the levels of active SR proteins. Our results further highlight the role for an nrRNA in the regulation of gene expression.
- University of Toronto Canada
- University of Illinois at Urbana–Champaign United States
- University of Illinois at Urbana Champaign United States
- Wright State University United States
- University System of Ohio United States
Cell Nucleus, Binding Sites, RNA, Untranslated, Mitosis, Nuclear Proteins, RNA-Binding Proteins, Cell Biology, Cell Line, DNA-Binding Proteins, Minor Histocompatibility Antigens, Alternative Splicing, Mice, RNA Precursors, Animals, Humans, Protein Interaction Domains and Motifs, RNA Splicing Factors, Intranuclear Space, Phosphorylation, Molecular Biology, HeLa Cells, Protein Binding
Cell Nucleus, Binding Sites, RNA, Untranslated, Mitosis, Nuclear Proteins, RNA-Binding Proteins, Cell Biology, Cell Line, DNA-Binding Proteins, Minor Histocompatibility Antigens, Alternative Splicing, Mice, RNA Precursors, Animals, Humans, Protein Interaction Domains and Motifs, RNA Splicing Factors, Intranuclear Space, Phosphorylation, Molecular Biology, HeLa Cells, Protein Binding
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