Delay in Synthesis of the 3′ Splice Site Promotes trans-Splicing of the Preceding 5′ Splice Site
Copyright policy )Delay in Synthesis of the 3′ Splice Site Promotes trans-Splicing of the Preceding 5′ Splice Site
Premessenger RNA (pre-mRNA) splicing can occur within an individual pre-mRNA (cis-splicing) or between separate pre-mRNAs (trans-splicing). Although a number of examples of mammalian trans-splicing have been reported, the molecular mechanisms involved are poorly understood. Here, we investigate the mechanisms of Sp1 pre-mRNA trans-splicing with human cells expressing modified Sp1 transgenes. We find that the presence of a long intron or the insertion of an RNA polymerase II pause site within an intron promotes trans-splicing. We also add examples of naturally occurring trans-splicing. We propose that Sp1 trans-splicing, and other examples of mammalian trans-splicing, are a consequence of low-frequency disruption of the normal mechanisms that couple transcription and splicing.
- Harvard University United States
- University of Tokyo Japan
Chromatin Immunoprecipitation, Time Factors, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Cell Biology, Exons, Models, Biological, Introns, Rats, Trans-Splicing, RNA Precursors, Animals, Humans, RNA Splice Sites, Transgenes, Molecular Biology, Cell Line, Transformed, Transcription Factors
Chromatin Immunoprecipitation, Time Factors, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Cell Biology, Exons, Models, Biological, Introns, Rats, Trans-Splicing, RNA Precursors, Animals, Humans, RNA Splice Sites, Transgenes, Molecular Biology, Cell Line, Transformed, Transcription Factors
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