Transcriptional stimulation of anthrax toxin receptors by anthrax edema toxin and Bacillus anthracis Sterne spore
Copyright policy )Transcriptional stimulation of anthrax toxin receptors by anthrax edema toxin and Bacillus anthracis Sterne spore
We used quantitative real-time RT-PCR to not only investigate the mRNA levels of anthrax toxin receptor 1 (ANTXR1) and 2 (ANTXR2) in the murine J774A.1 macrophage cells and different tissues of mice, but also evaluate the effect of anthrax edema toxin and Bacillus anthracis Sterne spores on the expression of mRNA of these receptors. The mRNA transcripts of both receptors were detected in J774A.1 cells and mouse tissues such as the lung, heart, kidney, spleen, stomach, jejunum, brain, skeleton muscle, and skin. The ANTXR2 mRNA level was significantly higher than that of ANTXR1 in J774A.1 cells and all tissues examined. The mRNA expression of both receptors in the lung was the highest among the tissues evaluated. Interestingly, the mRNA expression of both receptors in J774A.1 cells was upregulated by edema toxin. In addition, ANTXR mRNA expression in the lung was downregulated after subcutaneous inoculation of B. anthracis Sterne spores as well as after intranasal administration of anthrax toxin-based vaccine BioThrax. These results suggest that anthrax edema toxin and B. anthracis Sterne spore are involved in the ANTXR mRNA regulation in host cells.
- University of Rochester United States
Spores, Bacterial, Antigens, Bacterial, Receptors, Peptide, Reverse Transcriptase Polymerase Chain Reaction, Macrophages, Bacterial Toxins, Microfilament Proteins, Down-Regulation, Mice, Inbred Strains, Receptors, Cell Surface, Anthrax Vaccines, Cell Line, Anthrax, Mice, Gene Expression Regulation, Bacillus anthracis, Biomarkers, Tumor, Animals, Female, RNA, Messenger
Spores, Bacterial, Antigens, Bacterial, Receptors, Peptide, Reverse Transcriptase Polymerase Chain Reaction, Macrophages, Bacterial Toxins, Microfilament Proteins, Down-Regulation, Mice, Inbred Strains, Receptors, Cell Surface, Anthrax Vaccines, Cell Line, Anthrax, Mice, Gene Expression Regulation, Bacillus anthracis, Biomarkers, Tumor, Animals, Female, RNA, Messenger
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).12 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!