Activation of the rat scavenger receptor class B type I gene by PPARα
Copyright policy )Activation of the rat scavenger receptor class B type I gene by PPARα
Peroxisomal proliferator activated receptor alpha (PPARalpha) is activated by fibrate drugs which are known to protect against atherosclerosis. The present study examines the effects of PPARalpha on SR-BI expression. For this study, a rat SR-BI promoter-luciferase reporter gene construct was co-transfected into different cell lines with expression vectors that encode for PPARalpha+/-retinoic X receptor alpha (RXRalpha). PPARalpha/RXR increased the activity of the SR-BI promoter, an effect that was enhanced by clofibrate. Sequence analysis of the rat SR-BI promoter revealed the presence of a putative peroxisomal proliferator response element (PPRE) at bp -1,622. Electrophoretic mobility shift assays demonstrated that PPARalpha and RXRalpha are able to bind to the SR-BI PPRE motif. In addition, mutational analysis studies confirmed that this PPRE motif is responsible for the PPARalpha/RXRalpha-dependent activation of the rat SR-BI promoter in the cell lines examined.
- Florida Southern College United States
- University of South Florida United States
Retinoid X Receptor alpha, Anticholesteremic Agents, Amino Acid Motifs, Gene Expression, Sequence Analysis, DNA, Scavenger Receptors, Class B, Transfection, Cell Line, Rats, Gene Expression Regulation, Genes, Reporter, Animals, Humans, PPAR alpha, Clofibrate, Luciferases, Promoter Regions, Genetic, Plasmids
Retinoid X Receptor alpha, Anticholesteremic Agents, Amino Acid Motifs, Gene Expression, Sequence Analysis, DNA, Scavenger Receptors, Class B, Transfection, Cell Line, Rats, Gene Expression Regulation, Genes, Reporter, Animals, Humans, PPAR alpha, Clofibrate, Luciferases, Promoter Regions, Genetic, Plasmids
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