Abstract The silk fibroin (SF) is the main protein of silk fiber and one of the strongest natural biopolymers. SF is known suitable and potential candidate materials in biomedical applications due to several useful features including good biocompatibility, biodegradability, transparency, nontoxic, and minimal inflammatory reaction. To compete with the mechanical properties of the natural counterpart, regenerated silk fibroin have been reinforced with inorganic fillers such as graphene (G) by solution casting. SF-G composites film were investigated on their characterization by using tensile test attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), x- ray diffraction (XRD) and scanning electron microscopy (SEM). This study report that all of the SF-G composites films have induced the β-sheet thus increased the crystalline structure. The SF-G composites films also shows higher elongation at break than those of pure SF film due to the effect of glycerol and the reinforcing effect of graphene. The improved in mechanical properties further enabled potential as biomedical application and flexible electronic devices