A number of new anti-fouling paints claimed to be more friendly to the environment, have entered the market since prohibition of biocide containing paints have been enforced in many areas. Leakage waters from five new anti-fouling paints were tested for toxic effects to the macro algae Ceramium tenuicorne and Ceramium strictum and to the crustacean Nitocra spinipes. A banned copper and irgarol 1051 containing anti-fouling paint was used as reference. Five of the six paints tested were toxic to all or some of the organisms after two weeks of leakage with EC50 ranging from 0.08 to around 2% leakage water and LC50 ranging from 1.1% to 88%. The toxicity of leakage water from these paints was still high after 16 weeks. We conclude that these paints contain substances toxic to common organisms in the coastal Baltic ecosystem. A silicone based paint did not exhibit toxic effects to the two organisms. We recommend that biological tests should be used to identify the most harmful products before they are released on the market.