Development of pan-phlebovirus RT-PCR assay

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 2016 English Publisher:Elsevier BVJournal:Journal of Virological Methods, volume 232, pages 29-32 (issn: 0166-0934,

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Authors: Alexander S, Klimentov; Alexander M, Butenko; Natalia V, Khutoretskaya; Elena Yu, Shustova; Victor F, Larichev; Olga V, Isaeva; Galina G, Karganova; +2 Authors

doi: 10.1016/j.jviromet.2016.02.009

pmid: 26947398

Development of pan-phlebovirus RT-PCR assay

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Abstract

This study reports the pan-phlebovirus assay capable of detecting both sandfly/mosquito- and tick-borne phleboviruses. Sensitivity and specificity of the assay was verified using a panel of arboviruses. The RT-PCR assay is simple and sensitive, and thus well suited for screening of field samples.

Related Organizations

Lomonosov Moscow State University
Russian Federation
Chumakov Institute of Poliomyelitis and Viral Encephalitides
Russian Federation

Keywords

Phlebovirus, Molecular Diagnostic Techniques, Reverse Transcriptase Polymerase Chain Reaction, Virology, Humans, Bunyaviridae Infections, Sensitivity and Specificity

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