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Journal of Neuroscience Methods
Article . 2012
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Single electrode dynamic clamp with StdpC

Authors: Samu, David; Marra, Vincenzo; Kemenes, Ildiko; Crossley, Michael; Kemenes, György; Staras, Kevin; Nowotny, Thomas;

Single electrode dynamic clamp with StdpC

Abstract

Dynamic clamp is a powerful approach for electrophysiological investigations allowing researchers to introduce artificial electrical components into target neurons to simulate ionic conductances, chemical or electrotonic inputs or connections to other cells. Due to the rapidly changing and potentially large current injections during dynamic clamp, problematic voltage artifacts appear on the electrode used to inject dynamic clamp currents into a target neuron. Dynamic clamp experiments, therefore, typically use two separate electrodes in the same cell, one for recording membrane potential and one for injecting currents. The requirement for two independent electrodes has been a limiting factor for the use of dynamic clamp in applications where dual recordings of this kind are difficult or impossible to achieve. The recent development of an active electrode compensation (AEC) method has overcome some of these prior limitations, permitting artifact-free dynamic clamp experimentation with a single electrode. Here we describe an AEC method for the free dynamic clamp software StdpC. The AEC component of StdpC is the first such system implemented for the use of non-expert users and comes with a set of semi-automated configuration and calibration procedures that facilitate its use. We briefly introduce the AEC method and its implementation in StdpC and then validate it with an electronic model cell and in two different biological preparations.

Related Organizations
Keywords

Patch-Clamp Techniques, Neuroscience(all), Models, Neurological, Hippocampus, Capacitance compensation, Electric Impedance, Animals, Current injection artifacts, Electrodes, Basic Neuroscience, Cells, Cultured, Lymnaea, Neurons, Reproducibility of Results, Bridge balance, Digital compensation, Active electrode compensation, Ganglia, Invertebrate, Rats, Electrophysiology, Hybrid system, Dynamic clamp, Calibration, Electronics, Artifacts, Algorithms, Software

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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
16
Top 10%
Top 10%
Top 10%
Green
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