
Radiation damping on 600 and 800 MHz cryoprobes was investigated. The phase angle β between a vector 90° phase shifted to the precessing magnetization and the rf field induced in the coil was found to depend markedly on whether an FID was being acquired or not. The magnitude of the radiation damping field was sufficiently strong to restore 95% of the equilibrium water magnetization of a 90% H2O sample in a 5 mm sample tube within about 5 ms following a 165° pulse. This can be exploited in water flip-back versions of NOESY and TOCSY experiments of proteins, but care must be taken to limit the effect of the radiation damping field from the water on the Ha protons. Long water-selective pulses can be applied only following corrections. We developed a program for correcting pulse shapes if β is non-zero. The WATERGATE scheme is shown to be insensitive to imperfections introduced by radiation damping.
RF fields, Magnetic Resonance Spectroscopy, Radio Waves, dimethyl sulfoxide, WATERGATE, 2 propanol, Water flip-back, Damping, Magnetization, dyes, Keywords: Cryoprobe, 2-Propanol, Radiation damping, Electromagnetic Fields, TOCSY, Dimethyl Sulfoxide, Pulse shapes, Phase angles, Carbon Isotopes, Radiation, carbon, reagents, w Cryoprobe, Reproducibility of Results, Water, indicators, Phase shifted, Cold Temperature, markers and buffers, Indicators and Reagents, Sample tube, Protons, Algorithms, NOESY, proton
RF fields, Magnetic Resonance Spectroscopy, Radio Waves, dimethyl sulfoxide, WATERGATE, 2 propanol, Water flip-back, Damping, Magnetization, dyes, Keywords: Cryoprobe, 2-Propanol, Radiation damping, Electromagnetic Fields, TOCSY, Dimethyl Sulfoxide, Pulse shapes, Phase angles, Carbon Isotopes, Radiation, carbon, reagents, w Cryoprobe, Reproducibility of Results, Water, indicators, Phase shifted, Cold Temperature, markers and buffers, Indicators and Reagents, Sample tube, Protons, Algorithms, NOESY, proton
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