The oriental fruit fly (Bactrocera dorsalis) larvae may commonly experience a hypoxia microenvironment and have evolved the ability to survive in the low oxygen condition with some physiological and biochemical mechanisms. However, little is known about the response of B. dorsalis to hypoxia or anoxia. In this study, the effect of anoxia on the survival of B. dorsalis was investigated. The results showed that the B. dorsalis larvae were quite tolerant to anoxia conditions and can tolerate up to 24 h of anoxia exposure without a significant reduction in survival, 100% mortality was reached after 84 h of anoxia exposure. The cDNA of hypoxia inducible factor (HIF) 1α and HIF-1β is 2912 and 3618 bp in length, encoding 766 and 648 amino acid residues, respectively. Both HIF-1α and HIF-1β contain conserved basic helix-loop-helix (bHLH) domain and Per-Arnt-Sim (PAS) domain. HIF-1α can be induced by hypoxia, whereas HIF-1β expression was not significantly changed with the oxygen concentration. Three major heat shock proteins (Hsps) expression increased significantly during anoxia and recovery and Hsp70 was the most responsive to anoxia. Four superoxide dismutase (SOD) genes expression were also up-regulated during anoxia exposure. These data suggest that B. dorsalis has a strategy to induce HIF-1α and HIF-1-responsive genes to survive in the low oxygen condition.